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===Blood group analysis===
On 21 November, the results of Chikatilo's blood test again revealed his blood type to be type A and not type AB. Due to the amount of physical and circumstantial evidence investigators had thus far compiled, which indicated Chikatilo was indeed the murderer they had been pursuing, plus the fact that investigators had deduced the blood type of the murderer they had pursued using semen samples obtained from the clothing and bodies of fourteen of the victims as opposed to actual blood samples, investigators obtained a sample of Chikatilo's semen to test his blood type, the results of which confirmed that Chikatilo's semen was type AB, whereas his blood and saliva were type A.{{sfn|Cullen|1994|p=190}}{{refn|group=n|Investigators had received a circular in 1988 indicating that in extremely rare cases, a man's blood type may differ from his semen and saliva type. A [[serology]] expert would later state at Chikatilo's trial he was an example of this "extremely rare phenomenon" she termed "paradoxical secretion."{{sfn|Cullen|1994|p=149-150}} These findings have been openly dismissed by experts in serology and [[DNA profiling|DNA analysis]], who state
Throughout the questioning, Chikatilo repeatedly denied that he had committed the murders, although he did confess to molesting several of his pupils during his career as a teacher.{{sfn|Cullen|1994|p=187-188}} He also produced several written essays for Kostoyev which, although evasive regarding the actual murders, did reveal psychological symptoms consistent with those predicted by Dr. Bukhanovsky in the 1985 profile he had written for the investigators.<ref>{{cite news|last=Matthews|first=Owen|url=https://www.themoscowtimes.com/1999/01/30/a-killers-confidante-in-a-murderous-town-a280832|title=A Killer's Confidante In a Murderous Town|work=[[The Moscow Times]]|date=30 January 1999|access-date=6 November 2022}}</ref> The interrogation tactics used by Kostoyev may also have caused Chikatilo to become defensive; the informer sharing a KGB cell with the suspect reported to police that Chikatilo had informed him that Kostoyev had repeatedly asked him direct questions regarding the mutilations inflicted upon the victims.{{sfn|Cullen|1994|p=190}}
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