Phi X 174: Difference between revisions
Whosasking (talk | contribs) m fix markup |
m Journal cites:, added 1 DOI, using AWB (7751) |
||
| Line 6: | Line 6: | ||
| species = '''''phi X 174 phage''''' |
| species = '''''phi X 174 phage''''' |
||
}} |
}} |
||
The '''phi X 174''' (or '''phi X''') [[bacteriophage]] was the first DNA-based [[genome]] to be sequenced. This work was completed by [[Fred Sanger]] and his team in 1977.<ref>{{cite journal |author=Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M |title=Nucleotide sequence of bacteriophage phi X174 DNA |journal=Nature |date=1977-02-24 |volume=265 |issue=5596 | |
The '''phi X 174''' (or '''phi X''') [[bacteriophage]] was the first DNA-based [[genome]] to be sequenced. This work was completed by [[Fred Sanger]] and his team in 1977.<ref>{{cite journal |author=Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M |title=Nucleotide sequence of bacteriophage phi X174 DNA |journal=Nature |date=1977-02-24 |volume=265 |issue=5596 |pages=687–695 |pmid=870828 |doi=10.1038/265687a0}}</ref> In 1962, [[Walter Fiers]] had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.<ref>{{cite journal |author=Fiers W, Sinsheimer RL |title=The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure |journal=J. Mol. Biol. |volume=5 |pages=424–434 |year=1962}}</ref> |
||
This [[bacteriophage|phage]] has a very small amount of [[DNA]]. Phi X has 11 [[gene]]s in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The [[GC-content]] is 44% and 95% of nucleotides belong to coding genes. |
This [[bacteriophage|phage]] has a very small amount of [[DNA]]. Phi X has 11 [[gene]]s in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The [[GC-content]] is 44% and 95% of nucleotides belong to coding genes. |
||
Revision as of 10:26, 18 June 2011
| Phi X 174 | |
|---|---|
| Virus classification | |
| Group: | Group II (ssDNA)
|
| Family: | |
| Genus: | |
| Species: | phi X 174 phage
|
The phi X 174 (or phi X) bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.[1] In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.[2]
This phage has a very small amount of DNA. Phi X has 11 genes in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The GC-content is 44% and 95% of nucleotides belong to coding genes.
In 2003, it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.[3]
Phi X is regularly used as a positive control in DNA sequencing due to its relatively small genome size in comparison to other organisms and the extensive work that has been done on it.
See also
References
- ^ Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (1977-02-24). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687–695. doi:10.1038/265687a0. PMID 870828.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Fiers W, Sinsheimer RL (1962). "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure". J. Mol. Biol. 5: 424–434.
- ^ Smith, Hamilton O. (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences. 100 (26): 15440–15445. doi:10.1073/pnas.2237126100. PMID 14657399. Retrieved 2007-10-08.
{{cite journal}}: Unknown parameter|coauthors=ignored (|author=suggested) (help)
External links
 | This genetics article is a stub. You can help Wikipedia by expanding it. |
 | This virus-related article is a stub. You can help Wikipedia by expanding it. |