Bicinchoninic acid: Difference between revisions
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| Name = Bicinchoninic acid |
| Name = Bicinchoninic acid |
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| ImageFile = Structure of bicinchoninic acid.png |
| ImageFile = Structure of bicinchoninic acid.png |
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| ImageSize = 200px |
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| ImageName = |
| ImageName = |
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| PIN = [2,2′-Biquinoline]-4,4′-dicarboxylic acid |
| PIN = [2,2′-Biquinoline]-4,4′-dicarboxylic acid |
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| Solubility = Partially soluble in cold water, hot water{{vague|date=October 2013}} |
| Solubility = Partially soluble in cold water, hot water{{vague|date=October 2013}} |
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| MeltingPtC = 352 |
| MeltingPtC = 352 |
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| MeltingPt_notes = decomposes |
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| MeltingPt_note = decomposition |
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| BoilingPt = |
| BoilingPt = |
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| pKa = |
| pKa = |
Revision as of 01:57, 11 May 2021
Names | |
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Preferred IUPAC name
[2,2′-Biquinoline]-4,4′-dicarboxylic acid | |
Other names
Bicinchoninic acid
4,4′-Dicarboxy-2,2′-biquinoline 2,2'-Biquinoline-4,4'-dicarboxylic acid | |
Identifiers | |
3D model (JSmol)
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ChEMBL | |
ChemSpider | |
ECHA InfoCard | 100.013.628 |
PubChem CID
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UNII | |
CompTox Dashboard (EPA)
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Properties | |
C20H12N2O4 | |
Molar mass | 344.326 g·mol−1 |
Appearance | Cream colored powder |
Odor | Characteristic odor[vague] |
Melting point | 352 °C (666 °F; 625 K) decomposes |
Partially soluble in cold water, hot water[vague] | |
Hazards | |
Occupational safety and health (OHS/OSH): | |
Main hazards
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Will irritate eyes and mucous membranes. |
NFPA 704 (fire diamond) | |
Safety data sheet (SDS) | External MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Bicinchoninic acid /baɪsɪnkɔːnɪnɪk/ is a weak acid composed of two carboxylated quinoline rings.
Bicinchoninic is an organic compound with the formula (C9H5CO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.[1][2][3]
Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. Bicinchoninic acid is used to analyze to monitor cuprous ion presence due to its purple coloration via a biuret reactionIn this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[4]
References
- ^ "Measurement of Protein Using Bicinchoninic Acid". Analytical Biochemistry. 150 (1): 76–85. 1985. doi:10.1016/0003-2697(85)90442-7.
- ^ . doi:10.1016/0003-2697(89)90101-2.
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(help) - ^ . doi:10.1016/S0076-6879(09)63008-1.
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(help) - ^ Pierce BCA Protein Assay Kit